Trucks are now allowed on the Al Ain - Dubai road during off peak hours.
In a statement, the Integrated Transport Centre said the decision was taken to improve commercial movement between the Emirates.
Trucks must weigh under 45 tonnes and only use the E 611, Al Ain - Dubai road between 10:00 pm and 6:00 am.
Trucks are still not permitted to use Al Shiwayb Street towards northern emirates until the current construction work is completed.